PrimePCR™ SYBR® Green Assay: CYP4A11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0041500


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PrimePCR™ PreAmp for SYBR® Green Assay: CYP4A11, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CYP4A11, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0038778
Assay Design:		Intron-spanning
Chromosome Location:		1:47403751-47407081question
Amplicon Length:		200
Splice Variants Targeted:		ENST00000310638 ENST00000371905 ENST00000371904 ENST00000457840 ENST00000371890 ENST00000294337 ENST00000371891

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates medium-chain fatty acids such as laurate and myristate. [provided by RefSeq Jul 2008]

Gene Symbol:		CYP4A11
Gene Name:		cytochrome P450, family 4, subfamily A, polypeptide 11
Aliases:		CP4Y, CYP4A2, CYP4AII
RefSeq:		NC_000001.10 NG_007932.1 NT_032977.9
Ensembl:		ENSG00000187048
Entrez:		1579
Chromosome Mapping:		1p33

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998800
y-intercept	35.990000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.