PrimePCR™ SYBR® Green Assay: Serpina1e, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCEP0043529


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PrimePCR™ PreAmp for SYBR® Green Assay: Serpina1e, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Serpina1e, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0041313
Assay Design:		exonic
Chromosome Location:		12:103947036-103948016question
Amplicon Length:		146
Splice Variants Targeted:		ENSMUST00000179677 ENSMUST00000097653 ENSMUST00000130846 ENSMUST00000056925 ENSMUST00000105307 ENSMUST00000105306 ENSMUST00000025003 ENSMUST00000022665 ENSMUST00000025202 ENSMUST00000162964 ENSMUST00000159489 ENSMUST00000101024 ENSMUST00000076321 ENSMUST00000162539 ENSMUST00000107289 ENSMUST00000050757 ENSMUST00000095450 ENSMUST00000164454 ENSMUST00000078869 ENSMUST00000072876 ENSMUST00000085056 ENSMUST00000074051 ENSMUST00000122229 ENSMUST00000085054 ENSMUST00000080771 ENSMUST00000109986 ENSMUS

Gene Information

Description Not Available

Gene Symbol:		Serpina1e
Gene Name:		Serine (or cysteine) peptidase inhibitor, clade A, member 1E
Aliases:		Dom5, PI5, Spi1-5
RefSeq:		NC_000078.6 NT_039551.8
Ensembl:		ENSMUSG00000072849
Entrez:		20704
Chromosome Mapping:		12 E\|12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	36.870000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.