PrimePCR™ SYBR® Green Assay: H2-Q6, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCEP0043535


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PrimePCR™ PreAmp for SYBR® Green Assay: H2-Q6, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: H2-Q6, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0041319
Assay Design:		exonic
Chromosome Location:		17:35428273-35428362question
Amplicon Length:		60
Splice Variants Targeted:		ENSMUST00000031446 ENSMUST00000076119 ENSMUST00000081435 ENSMUST00000174699 ENSMUST00000113879 ENSMUST00000054553 ENSMUST00000112428 ENSMUST00000112427 ENSMUST00000088308 ENSMUST00000112425 ENSMUST00000084275 ENSMUST00000112424 ENSMUST00000046064 ENSMUST00000110909 ENSMUST00000172912 ENSMUST00000025181 ENSMUST00000087189 ENSMUST00000172760 ENSMUST00000174067 ENSMUST00000172819 ENSMUST00000173674

Gene Information

Description Not Available

Gene Symbol:		H2-Q6
Gene Name:		Histocompatibility 2, Q region locus 6
Aliases:		0610037M15Rik, H-2Q6, Qa-6, Qa6
RefSeq:		NC_000083.6 NT_039649.8 NT_039662.3 NT_187004.1 NT_187027.1
Ensembl:		ENSMUSG00000073409
Entrez:		110557
Chromosome Mapping:		17 B1\|17 19.18 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	38.290000
Efficiency	88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.