PrimePCR™ SYBR® Green Assay: Cyb5r3, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


List Price:		$179.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
Add to My PrimePCR Add to My PrimePCR		Add to Quote Add to Quote
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Cyb5r3, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

List Price:		$174.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
[ Add to My PrimePCR ] [ Add to My PrimePCR ]		[ Add to Quote ] [ Add to Quote ]

PrimePCR™ Template for SYBR® Green Assay: Cyb5r3, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

List Price:		$179.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
[ Add to My PrimePCR ] [ Add to My PrimePCR ]		[ Add to Quote ] [ Add to Quote ]

Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCID0008930
Assay Design:		intron_spanning
Chromosome Location:		7:124014727-124018178question
Amplicon Length:		115
Splice Variants Targeted:		ENSRNOT00000000559 ENSRNOT00000064700 ENSRNOT00000012878

Gene Information

catalyzes the conversion of NADH, H+, and 2 ferricytochrome b(5) to NAD+ and 2 ferrocytochrome b(5) [RGD, Feb 2006]

Gene Symbol:		Cyb5r3
Gene Name:		NADH-cytochrome b5 reductase 3 NADH-cytochrome b5 reductase 3 membrane-bound form NADH-cytochrome b5 reductase 3 soluble form
Aliases:		Not Available
RefSeq:		NM_138877
Ensembl:		ENSRNOG00000009592
Entrez:		25035
Chromosome Mapping:		7q34

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	35.430000
Efficiency	103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.