PrimePCR™ SYBR® Green Assay: Clock, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Clock, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Clock, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0009227
Assay Design:   intron_spanning
Chromosome Location:   14:34268432-34271899question
Amplicon Length:   112
Splice Variants Targeted:   ENSRNOT00000002976

Gene Information

The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with Arntl (Bmal1) that binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Polymorphisms in this gene may be associated with behavioral changes, obesity, and metabolic syndrome. Two transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]

Gene Symbol:   Clock
Gene Name:   Circadian locomoter output cycles protein kaput
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000002175
Entrez:   60447
Chromosome Mapping:   14p11

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