PrimePCR™ Probe Assay: SCNN1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SCNN1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SCNN1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024081
Assay Design:   Exonic
Chromosome Location:   12:6456234-6456400question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000360168 ENST00000540037 ENST00000228916 ENST00000358945

Gene Information

Nonvoltage-gated amiloride-sensitive sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha beta and gamma. This gene encodes the alpha subunit and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1) a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Apr 2009]

Gene Symbol:   SCNN1A
Gene Name:   sodium channel, nonvoltage-gated 1 alpha
Aliases:   BESC2, ENaCa, ENaCalpha, FLJ21883, SCNEA, SCNN1
RefSeq:   NC_000012.11 NG_007506.1 NG_011945.1 NT_009759.16
Ensembl:   ENSG00000111319
Entrez:   6337
Chromosome Mapping:   12p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.440000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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