PrimePCR™ Probe Assay: ATXN3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ATXN3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATXN3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040656
Assay Design:   exonic
Chromosome Location:   14:92537279-92537368question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000542433 ENST00000304636 ENST00000429658 ENST00000309558 ENST00000481100 ENST00000393287 ENST00000502250 ENST00000503767 ENST00000340660 ENST00000532032 ENST00000555381 ENST00000557311 ENST00000554592 ENST00000554672 ENST00000553491 ENST00000556220 ENST00000545170 ENST00000429774 ENST00000237449 ENST00000389394 ENST00000398278

Gene Information

Machado-Joseph disease also known as spinocerebellar ataxia-3 is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region and the expansion of these repeats from the normal 13-36 to 68-79 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Sep 2009]

Gene Symbol:   ATXN3
Gene Name:   ataxin 3
Aliases:   AT3, ATX3, JOS, MJD, MJD1, SCA3
RefSeq:   NC_000014.8 NT_026437.12 NG_008198.1
Ensembl:   ENSG00000066427
Entrez:   4287
Chromosome Mapping:   14q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.060000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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