PrimePCR™ Probe Assay: SEMA3A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SEMA3A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SEMA3A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041232
Assay Design:   exonic
Chromosome Location:   7:83823896-83823989question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000265362 ENST00000436949 ENST00000420047 ENST00000448879 ENST00000424555

Gene Information

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent inhibiting axonal outgrowth or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq Jul 2008]

Gene Symbol:   SEMA3A
Gene Name:   sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Aliases:   Hsema-I, Hsema-III, MGC133243, SEMA1, SEMAD, SEMAIII, SEMAL, SemD, coll-1
RefSeq:   NC_000007.13 NG_011489.1 NT_007933.15
Ensembl:   ENSG00000075213
Entrez:   10371
Chromosome Mapping:   7p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.130000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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