PrimePCR™ Probe Assay: HSPA8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HSPA8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HSPA8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041393
Assay Design:   exonic
Chromosome Location:   11:122931409-122931835question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000532636 ENST00000533540 ENST00000534624 ENST00000453788 ENST00000227378 ENST00000526110 ENST00000528292 ENST00000525624 ENST00000534567 ENST00000527387 ENST00000532182 ENST00000524590 ENST00000530391

Gene Information

This gene encodes a member of the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group which are also referred to as heat-shock cognate proteins. It functions as a chaperone and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   HSPA8
Gene Name:   heat shock 70kDa protein 8
Aliases:   HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP1, MGC131511, MGC29929, NIP71
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000109971
Entrez:   3312
Chromosome Mapping:   11q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.910000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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