PrimePCR™ Probe Assay: TIE1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TIE1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TIE1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049494
Assay Design:   exonic
Chromosome Location:   1:43770712-43770923question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000377813 ENST00000360807 ENST00000377807 ENST00000246043 ENST00000455029 ENST00000538015 ENST00000441333 ENST00000372476

Gene Information

This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Nov 2011]

Gene Symbol:   TIE1
Gene Name:   tyrosine kinase with immunoglobulin-like and EGF-like domains 1
Aliases:   JTK14, TIE
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000066056
Entrez:   7075
Chromosome Mapping:   1p34-p33

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