PrimePCR™ Probe Assay: UBE2D1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: UBE2D1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: UBE2D1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049581
Assay Design:   exonic
Chromosome Location:   10:60128628-60128766question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000373910

Gene Information

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes or E1s ubiquitin-conjugating enzymes or E2s and ubiquitin-protein ligases or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT) and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2011]

Gene Symbol:   UBE2D1
Gene Name:   ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
Aliases:   E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000072401
Entrez:   7321
Chromosome Mapping:   10q21.1

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