PrimePCR™ Probe Assay: TBX21, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TBX21, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TBX21, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049606
Assay Design:   exonic
Chromosome Location:   17:45822708-45822809question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000177694

Gene Information

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq Jul 2008]

Gene Symbol:   TBX21
Gene Name:   T-box 21
Aliases:   T-PET, T-bet, TBET, TBLYM
RefSeq:   NC_000017.10 NG_012166.1 NT_010783.15
Ensembl:   ENSG00000073861
Entrez:   30009
Chromosome Mapping:   17q21.32

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