PrimePCR™ Probe Assay: MLH1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MLH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MLH1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049644
Assay Design:   exonic
Chromosome Location:   3:37070305-37081680question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000231790 ENST00000456676 ENST00000458205 ENST00000455445 ENST00000435176 ENST00000450420 ENST00000413740 ENST00000536378 ENST00000539477

Gene Information

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but their full-length natures have not been determined.[provided by RefSeq Nov 2009]

Gene Symbol:   MLH1
Gene Name:   mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
Aliases:   COCA2, FCC2, HNPCC, HNPCC2, MGC5172, hMLH1
RefSeq:   NC_000003.11 NG_007109.1 NG_008418.1 NT_022517.18
Ensembl:   ENSG00000076242
Entrez:   4292
Chromosome Mapping:   3p21.3

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