PrimePCR™ Probe Assay: MSH2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MSH2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MSH2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049938
Assay Design:   exonic
Chromosome Location:   2:47637293-47637393question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000233146 ENST00000454849 ENST00000406134 ENST00000543555

Gene Information

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned it was discovered to be a human homolog of the E. coli mismatch repair gene mutS consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. [provided by RefSeq Jul 2008]

Gene Symbol:   MSH2
Gene Name:   mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Aliases:   COCA1, FCC1, HNPCC, HNPCC1, LCFS2
RefSeq:   NC_000002.11 NG_007110.1 NT_022184.15
Ensembl:   ENSG00000095002
Entrez:   4436
Chromosome Mapping:   2p21

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