PrimePCR™ Probe Assay: PGK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PGK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PGK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050174
Assay Design:   exonic
Chromosome Location:   X:77380922-77381435question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000373316 ENST00000442431 ENST00000537456 ENST00000597340 ENST00000593375 ENST00000593893

Gene Information

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 13-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq Oct 2008]

Gene Symbol:   PGK1
Gene Name:   phosphoglycerate kinase 1
Aliases:   MGC117307, MGC142128, MGC8947, MIG10, PGKA
RefSeq:   NC_000023.10 NG_008862.1 NT_011651.17
Ensembl:   ENSG00000102144
Entrez:   5230
Chromosome Mapping:   Xq13

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