PrimePCR™ Probe Assay: ZAP70, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZAP70, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZAP70, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050836
Assay Design:   exonic
Chromosome Location:   2:98351058-98351148question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000442208 ENST00000451498 ENST00000264972 ENST00000234313

Gene Information

This gene encodes an enzyme belonging to the protein tyrosine kinase family and it plays a role in T-cell development and lymphocyte activation. This enzyme which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ZAP70
Gene Name:   zeta-chain (TCR) associated protein kinase 70kDa
Aliases:   FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70
RefSeq:   NC_000002.11 NG_007727.1 NT_022171.15
Ensembl:   ENSG00000115085
Entrez:   7535
Chromosome Mapping:   2q12

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