PrimePCR™ Probe Assay: HTRA2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HTRA2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HTRA2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050862
Assay Design:   exonic
Chromosome Location:   2:74757545-74757753question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000437202 ENST00000352222 ENST00000258080

Gene Information

This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described but their full-length sequences have not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   HTRA2
Gene Name:   HtrA serine peptidase 2
Aliases:   OMI, PARK13, PRSS25
RefSeq:   NC_000002.11 NG_012163.1 NT_022184.15
Ensembl:   ENSG00000115317
Entrez:   27429
Chromosome Mapping:   2p12

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