PrimePCR™ Probe Assay: CCT4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CCT4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CCT4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050879
Assay Design:   exonic
Chromosome Location:   2:62099284-62099432question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000394440 ENST00000544079 ENST00000538252 ENST00000544185

Gene Information

The chaperonin containing TCP1 (MIM 186980) complex (CCT) also called the TCP1 ring complex consists of 2 back-to-back rings each containing 8 unique but homologous subunits such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130) as well as alpha-transducin (MIM 139330) (Won et al. 1998 [PubMed 9819444]).[supplied by OMIM Mar 2008]

Gene Symbol:   CCT4
Gene Name:   chaperonin containing TCP1, subunit 4 (delta)
Aliases:   CCT-DELTA, Cctd, MGC126164, MGC126165, SRB
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000115484
Entrez:   10575
Chromosome Mapping:   2p15

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