PrimePCR™ Probe Assay: PIN1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PIN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PIN1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051446
Assay Design:   exonic
Chromosome Location:   19:9949233-9949323question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000332797 ENST00000431948 ENST00000445054 ENST00000265136 ENST00000395542 ENST00000349129 ENST00000369739 ENST00000237163 ENST00000322002 ENST00000238667 ENST00000378495 ENST00000576778 ENST00000356205 ENST00000368623 ENST00000368621 ENST00000310483 ENST00000429040 ENST00000413622 ENST00000537590 ENST00000311277 ENST00000307063 ENST00000587625 ENST00000247970 ENST00000588695 ENST00000536376 ENST00000395503 ENST00000357084

Gene Information

Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth genotoxic and other stress responses the immune response induction and maintenance of pluripotency germ cell development neuronal differentiation and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq Jun 2011]

Gene Symbol:   PIN1
Gene Name:   peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Aliases:   DOD, UBL5
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000127445
Entrez:   5300
Chromosome Mapping:   19p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.520000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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