PrimePCR™ Probe Assay: AKAP12, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AKAP12, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AKAP12, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051616
Assay Design:   exonic
Chromosome Location:   6:151672223-151672337question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000253332 ENST00000354675 ENST00000402676 ENST00000359755

Gene Information

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells cultured fibroblasts and osteosarcoma cells. It associates with protein kinases A and C and phosphatase and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   AKAP12
Gene Name:   A kinase (PRKA) anchor protein 12
Aliases:   AKAP250, DKFZp686M0430, DKFZp686O0331, FLJ20945, FLJ97621
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000131016
Entrez:   9590
Chromosome Mapping:   6q24-q25

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