PrimePCR™ Probe Assay: NUMB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NUMB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NUMB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051778
Assay Design:   exonic
Chromosome Location:   14:73822359-73822451question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000554546 ENST00000356296 ENST00000557597 ENST00000555238 ENST00000555394 ENST00000559312 ENST00000560335 ENST00000555738 ENST00000554521 ENST00000555859 ENST00000555307 ENST00000554394 ENST00000555987 ENST00000554818 ENST00000355058 ENST00000359560 ENST00000454166 ENST00000544991 ENST00000535282

Gene Information

The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein whose degradation is induced in a proteasome-dependent manner by MDM2 is a membrane-bound protein that has been shown to associate with EPS15 LNX1 and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   NUMB
Gene Name:   numb homolog (Drosophila)
Aliases:   S171
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000133961
Entrez:   8650
Chromosome Mapping:   14q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.730000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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