PrimePCR™ Probe Assay: IL10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: IL10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051966
Assay Design:   exonic
Chromosome Location:   1:206941904-206942019question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000423557

Gene Information

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines MHC class II Ags and costimulatory molecules on macrophages. It also enhances B cell survival proliferation and antibody production. This cytokine can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq May 2011]

Gene Symbol:   IL10
Gene Name:   interleukin 10
Aliases:   CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF
RefSeq:   NC_000001.10 NG_012088.1 NT_167186.1
Ensembl:   ENSG00000136634
Entrez:   3586
Chromosome Mapping:   1q31-q32

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