PrimePCR™ Probe Assay: XPC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: XPC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: XPC, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052912
Assay Design:   exonic
Chromosome Location:   3:14211969-14214381question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000285021 ENST00000511155 ENST00000449060

Gene Information

This gene encodes a component of the nucleotide excision repair (NER) pathway. There are multiple components involved in the NER pathway including Xeroderma pigmentosum (XP) A-G and V Cockayne syndrome (CS) A and B and trichothiodystrophy (TTD) group A etc. This component XPC plays an important role in the early steps of global genome NER especially in damage recognition open complex formation and repair protein complex formation. Mutations in this gene or some other NER components result in Xeroderma pigmentosum a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:   XPC
Gene Name:   xeroderma pigmentosum, complementation group C
Aliases:   RAD4, XP3, XPCC
RefSeq:   NC_000003.11 NG_008975.1 NT_022517.18 NG_011763.1
Ensembl:   ENSG00000154767
Entrez:   7508
Chromosome Mapping:   3p25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.400000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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