PrimePCR™ Probe Assay: RELA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RELA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RELA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054212
Assay Design:   exonic
Chromosome Location:   11:65429428-65429652question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000406246 ENST00000525693 ENST00000308639 ENST00000532999 ENST00000534558 ENST00000527749 ENST00000532879 ENST00000533187 ENST00000527874 ENST00000534283

Gene Information

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL RELA or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   RELA
Gene Name:   v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Aliases:   MGC131774, NFKB3, p65
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000173039
Entrez:   5970
Chromosome Mapping:   11q13

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