PrimePCR™ Probe Assay: NKX2-5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NKX2-5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NKX2-5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054834
Assay Design:   exonic
Chromosome Location:   5:172661789-172661882question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000551577 ENST00000329198 ENST00000424406 ENST00000521848 ENST00000517440

Gene Information

This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect and also tetralogy of Fallot which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5 a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2009]

Gene Symbol:   NKX2-5
Gene Name:   NK2 transcription factor related, locus 5 (Drosophila)
Aliases:   CHNG5, CSX, CSX1, FLJ52202, FLJ97166, FLJ97195, FLJ97197, FLJ99536, NKX2.5, NKX2E, NKX4-1
RefSeq:   NC_000005.9 NT_023133.13 NG_013340.1
Ensembl:   ENSG00000183072
Entrez:   1482
Chromosome Mapping:   5q34

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