PrimePCR™ Probe Assay: HIST1H4A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HIST1H4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HIST1H4A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055310
Assay Design:   exonic
Chromosome Location:   6:26021965-26022182question
Amplicon Length:   188
Splice Variants Targeted:   ENST00000359907 ENST00000377364 ENST00000346706 ENST00000405109 ENST00000407609 ENST00000343941 ENST00000405256 ENST00000377803 ENST00000340756 ENST00000360441 ENST00000377745 ENST00000342992 ENST00000460472 ENST00000589042 ENST00000591111 ENST00000342175 ENST00000359218 ENST00000354348 ENST00000355057 ENST00000357549 ENST00000355981 ENST00000369165 ENST00000392939 ENST00000392938 ENST00000392933 ENST00000369157 ENST00000392932 ENST00000411726 ENST00000242375 ENST00000539745 ENST00000578186 ENST

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A H2B H3 and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq Jul 2008]

Gene Symbol:   HIST1H4A
Gene Name:   histone cluster 1, H4a
Aliases:   H4/a, H4FA
RefSeq:   NC_000006.11 NG_001335.1 NT_007592.15
Ensembl:   ENSG00000196176
Entrez:   8359
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.170000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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