PrimePCR™ Probe Assay: MDH1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MDH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MDH1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057510
Assay Design:   exonic
Chromosome Location:   2:63824701-63826391question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000373477 ENST00000233114 ENST00000409476 ENST00000436321 ENST00000432309 ENST00000539945 ENST00000394423 ENST00000544381

Gene Information

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq Nov 2010]

Gene Symbol:   MDH1
Gene Name:   malate dehydrogenase 1, NAD (soluble)
Aliases:   MDH-s, MDHA, MGC:1375, MOR2
RefSeq:   NG_028144.1 NC_000002.11 NT_022184.15
Ensembl:   ENSG00000014641
Entrez:   4190
Chromosome Mapping:   2p13.3

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