PrimePCR™ Probe Assay: ITGB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ITGB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ITGB2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057618
Assay Design:   exonic
Chromosome Location:   21:46306170-46306314question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000397852 ENST00000397857 ENST00000397854 ENST00000355153 ENST00000397850 ENST00000302347

Gene Information

The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example beta 2 combines with the alpha L chain to form the integrin LFA-1 and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ITGB2
Gene Name:   integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Aliases:   CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7
RefSeq:   NC_000021.8 NG_007270.2 NT_011515.12
Ensembl:   ENSG00000160255
Entrez:   3689
Chromosome Mapping:   21q22.3

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