PrimePCR™ Probe Assay: IL4R, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL4R, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL4R, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057752
Assay Design:   exonic
Chromosome Location:   16:27375473-27375614question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000395762 ENST00000543915 ENST00000565352 ENST00000170630

Gene Information

This gene encodes the alpha chain of the interleukin-4 receptor a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy a condition that can manifest itself as allergic rhinitis sinusitus asthma or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. [provided by RefSeq Apr 2012]

Gene Symbol:   IL4R
Gene Name:   interleukin 4 receptor
Aliases:   CD124, IL4RA
RefSeq:   NC_000016.9 NG_012086.1 NT_010393.16
Ensembl:   ENSG00000077238
Entrez:   3566
Chromosome Mapping:   16p12.1-p11.2

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