PrimePCR™ Probe Assay: GMNN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GMNN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GMNN, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057753
Assay Design:   exonic
Chromosome Location:   6:24786098-24786246question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000356509 ENST00000230056

Gene Information

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1 preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq Oct 2011]

Gene Symbol:   GMNN
Gene Name:   geminin, DNA replication inhibitor
Aliases:   Gem, RP3-369A17.3
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000112312
Entrez:   51053
Chromosome Mapping:   6p22.3

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