PrimePCR™ Probe Assay: RBPJ, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RBPJ, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RBPJ, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057906
Assay Design:   exonic
Chromosome Location:   4:26436233-26436346question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000342320

Gene Information

The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Also this protein can bind specifically to the recombination signal sequence of immunglobulin kappa type J segments. Several transcript variants encoding different isoforms have been found for this gene and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq Oct 2011]

Gene Symbol:   RBPJ
Gene Name:   recombination signal binding protein for immunoglobulin kappa J region
Aliases:   CBF1, IGKJRB, IGKJRB1, KBF2, MGC61669, RBP-J, RBPJK, RBPSUH, SUH, csl
RefSeq:   NC_000004.11 NT_006316.16
Ensembl:   ENSG00000168214
Entrez:   3516
Chromosome Mapping:   4p15.2

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