PrimePCR™ Probe Assay: BCL2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BCL2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BCL2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058350
Assay Design:   exonic
Chromosome Location:   18:60985331-60985468question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000398117 ENST00000333681 ENST00000589955 ENST00000444484

Gene Information

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2 such as in the case of translocation of BCL2 to Ig heavy chain locus is thought to be the cause of follicular lymphoma. Two transcript variants produced by alternate splicing differ in their C-terminal ends. [provided by RefSeq Jul 2008]

Gene Symbol:   BCL2
Gene Name:   B-cell CLL/lymphoma 2
Aliases:   Bcl-2
RefSeq:   NC_000018.9 NG_009361.1 NT_025028.14
Ensembl:   ENSG00000171791
Entrez:   596
Chromosome Mapping:   18q21.33,18q21.3

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