PrimePCR™ Probe Assay: KPNA6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KPNA6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KPNA6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026223
Assay Design:   Intron-spanning
Chromosome Location:   1:32626244-32627597question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000373625 ENST00000446515 ENST00000545542 ENST00000537234 ENST00000373617

Gene Information

Nucleocytoplasmic transport a signal- and energy-dependent process takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. The protein encoded by this gene is a member of the importin alpha family. [provided by RefSeq Jul 2008]

Gene Symbol:   KPNA6
Gene Name:   karyopherin alpha 6 (importin alpha 7)
Aliases:   FLJ11249, IPOA7, KPNA7, MGC17918
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000025800
Entrez:   23633
Chromosome Mapping:   1p35.1

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