PrimePCR™ Probe Assay: SERPINB8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SERPINB8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SERPINB8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026246
Assay Design:   Intron-spanning
Chromosome Location:   18:61647096-61649033question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000397985 ENST00000397988 ENST00000441827 ENST00000295211 ENST00000353706 ENST00000542677

Gene Information

The superfamily of high molecular weight serine proteinase inhibitors (serpins) regulate a diverse set of intracellular and extracellular processes such as complement activation fibrinolysis coagulation cellular differentiation tumor suppression apoptosis and cell migration. Serpins are characterized by well-conserved a tertiary structure that consists of 3 beta sheets and 8 or 9 alpha helices (Huber and Carrell 1989 [PubMed 2690952]). A critical portion of the molecule the reactive center loop connects beta sheets A and C. Protease inhibitor-8 (PI8; SERPINB8) is a member of the ov-serpin subfamily which relative to the archetypal serpin PI1 (MIM 107400) is characterized by a high degree of homology to chicken ovalbumin lack of N- and C-terminal extensions absence of a signal peptide and a serine rather than an asparagine residue at the penultimate position (summary by Bartuski et al. 1997 [PubMed 9268635]).[supplied by OMIM Jan 2010]

Gene Symbol:   SERPINB8
Gene Name:   serpin peptidase inhibitor, clade B (ovalbumin), member 8
Aliases:   CAP2, PI8
RefSeq:   NC_000018.9 NT_025028.14
Ensembl:   ENSG00000166401
Entrez:   5271
Chromosome Mapping:   18q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.720000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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