PrimePCR™ Probe Assay: SP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026675
Assay Design:   Intron-spanning
Chromosome Location:   12:53803310-53804777question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000426431 ENST00000327443

Gene Information

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes including cell differentiation cell growth apoptosis immune responses response to DNA damage and chromatin remodeling. Post-translational modifications such as phosphorylation acetylation glycosylation and proteolytic processing significantly affect the activity of this protein which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   SP1
Gene Name:   Sp1 transcription factor
Aliases:   Not Available
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000185591
Entrez:   6667
Chromosome Mapping:   12q13.1

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