PrimePCR™ Probe Assay: LMNB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LMNB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LMNB2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027822
Assay Design:   Intron-spanning
Chromosome Location:   19:2430903-2431642question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000325327

Gene Information

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability chromatin structure and gene expression. Vertebrate lamins consist of two types A and B. This gene encodes one of the two B type proteins B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LMNB2
Gene Name:   lamin B2
Aliases:   LAMB2, LMN2, MGC2721
RefSeq:   NC_000019.9 NG_008355.1 NT_011255.14
Ensembl:   ENSG00000176619
Entrez:   84823
Chromosome Mapping:   19p13.3

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