PrimePCR™ Probe Assay: RACGAP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RACGAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RACGAP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027960
Assay Design:   Intron-spanning
Chromosome Location:   12:50399113-50400284question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000427314 ENST00000312377 ENST00000454520 ENST00000551016 ENST00000547905 ENST00000552310 ENST00000546595 ENST00000551145 ENST00000548824 ENST00000548644 ENST00000546723 ENST00000552921 ENST00000552157 ENST00000546764 ENST00000551876 ENST00000549777 ENST00000550651 ENST00000434422

Gene Information

The protein encoded by this gene belongs to the GTPase-activating protein (GAP) family. GAPs bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function GAPs negatively regulate Rho-mediated signals. This protein plays a regulatory role in initiation of cytokinesis controlling cell growth and differentiation of hematopoietic cells regulating spermatogenesis and in neuronal proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   RACGAP1
Gene Name:   Rac GTPase activating protein 1
Aliases:   HsCYK-4, ID-GAP, MgcRacGAP
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000161800
Entrez:   29127
Chromosome Mapping:   12q13.12

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