PrimePCR™ Probe Assay: PCNA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PCNA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PCNA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028602
Assay Design:   Intron-spanning
Chromosome Location:   20:5096158-5098175question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000379143 ENST00000379160

Gene Information

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq Jul 2008]

Gene Symbol:   PCNA
Gene Name:   proliferating cell nuclear antigen
Aliases:   MGC8367
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000132646
Entrez:   5111
Chromosome Mapping:   20pter-p12

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