PrimePCR™ Probe Assay: ALDOB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ALDOB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ALDOB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028624
Assay Design:   Intron-spanning
Chromosome Location:   9:104187794-104188868question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000374855 ENST00000374853 ENST00000430164

Gene Information

Fructose-16-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-16-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A B and C are distinct proteins the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver kidney and intestine aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq Dec 2008]

Gene Symbol:   ALDOB
Gene Name:   aldolase B, fructose-bisphosphate
Aliases:   ALDB, ALDO2
RefSeq:   NC_000009.11 NG_012387.1 NT_008470.19
Ensembl:   ENSG00000136872
Entrez:   229
Chromosome Mapping:   9q21.3-q22.2

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