PrimePCR™ Probe Assay: CD46, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CD46, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CD46, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028845
Assay Design:   Intron-spanning
Chromosome Location:   1:207925614-207930451question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000358170 ENST00000354848 ENST00000322918 ENST00000367042 ENST00000367041 ENST00000357714 ENST00000322875 ENST00000360212 ENST00000480003 ENST00000441839 ENST00000361067

Gene Information

The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I which protects the host cell from damage by complement. In addition the encoded protein can act as a receptor for the Edmonston strain of measles virus human herpesvirus-6 and type IV pili of pathogenic Neisseria. Finally the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jun 2010]

Gene Symbol:   CD46
Gene Name:   CD46 molecule, complement regulatory protein
Aliases:   AHUS2, MCP, MGC26544, MIC10, TLX, TRA2.10
RefSeq:   NC_000001.10 NG_009296.1 NT_167186.1
Ensembl:   ENSG00000117335
Entrez:   4179
Chromosome Mapping:   1q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.530000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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