PrimePCR™ Probe Assay: GSR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GSR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GSR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030143
Assay Design:   Intron-spanning
Chromosome Location:   8:30560747-30567403question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000221130 ENST00000520888 ENST00000521479 ENST00000546342 ENST00000541648 ENST00000537535 ENST00000414019

Gene Information

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq Aug 2010]

Gene Symbol:   GSR
Gene Name:   glutathione reductase
Aliases:   MGC78522
RefSeq:   NC_000008.10 NG_027719.1 NT_167187.1
Ensembl:   ENSG00000104687
Entrez:   2936
Chromosome Mapping:   8p21.1

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