PrimePCR™ Probe Assay: CACNA1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CACNA1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CACNA1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030898
Assay Design:   Intron-spanning
Chromosome Location:   19:13370404-13372302question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000445042 ENST00000360228 ENST00000418012 ENST00000357018 ENST00000325084

Gene Information

Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes including muscle contraction hormone or neurotransmitter release and gene expression. Calcium channels are multisubunit complexes composed of alpha-1 beta alpha-2/delta and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit whereas the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms alpha-1A B C D E and S. This gene encodes the alpha-1A subunit which is predominantly expressed in neuronal tissue. Mutations in this gene are associated with 2 neurologic disorders familial hemiplegic migraine and episodic ataxia 2. This gene also exhibits polymorphic variation due to (CAG)n-repeats. Multiple transcript variants encoding different isoforms have been found for this gene. In one set of transcript variants the (CAG)n-repeats occur in the 3' UTR and are not associated with any disease. But in another set of variants an insertion extends the coding region to include the (CAG)n-repeats which encode a polyglutamine tract. Expansion of the (CAG)n-repeats from the normal 4-16 to 21-28 in the coding region is associated with spinocerebellar ataxia 6. [provided by RefSeq Mar 2010]

Gene Symbol:   CACNA1A
Gene Name:   calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
Aliases:   APCA, CACNL1A4, CAV2.1, EA2, FHM, HPCA, MHP, MHP1, SCA6
RefSeq:   NC_000019.9 NT_011295.11 NG_011569.1
Ensembl:   ENSG00000141837
Entrez:   773
Chromosome Mapping:   19p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 34.540000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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