PrimePCR™ Probe Assay: GPX3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GPX3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GPX3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0032674
Assay Design:   Intron-spanning
Chromosome Location:   5:150406480-150407035question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000388825 ENST00000521650 ENST00000517973 ENST00000458211

Gene Information

This gene product belongs to the glutathione peroxidase family which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq Jul 2008]

Gene Symbol:   GPX3
Gene Name:   glutathione peroxidase 3 (plasma)
Aliases:   GPx-P, GSHPx-3, GSHPx-P
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000211445
Entrez:   2878
Chromosome Mapping:   5q23

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