PrimePCR™ Probe Assay: SPI1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SPI1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SPI1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033288
Assay Design:   Intron-spanning
Chromosome Location:   11:47397227-47399931question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000378538 ENST00000227163 ENST00000533968

Gene Information

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SPI1
Gene Name:   spleen focus forming virus (SFFV) proviral integration oncogene spi1
Aliases:   OF, PU.1, SFPI1, SPI-1, SPI-A
RefSeq:   NC_000011.9 NG_007667.1 NT_009237.18
Ensembl:   ENSG00000066336
Entrez:   6688
Chromosome Mapping:   11p11.2

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