PrimePCR™ Probe Assay: BRAF, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BRAF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BRAF, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033856
Assay Design:   Intron-spanning
Chromosome Location:   7:140534615-140549990question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000288602

Gene Information

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway which affects cell division differentiation and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome a disease characterized by heart defects mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers including non-Hodgkin lymphoma colorectal cancer malignant melanoma thyroid carcinoma non-small cell lung carcinoma and adenocarcinoma of lung. A pseudogene which is located on chromosome X has been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   BRAF
Gene Name:   v-raf murine sarcoma viral oncogene homolog B1
Aliases:   B-RAF1, BRAF1, FLJ95109, MGC126806, MGC138284, NS7, RAFB1
RefSeq:   NC_000007.13 NG_007873.1 NT_007914.15
Ensembl:   ENSG00000157764
Entrez:   673
Chromosome Mapping:   7q34

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