PrimePCR™ Probe Assay: HSP90B1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HSP90B1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HSP90B1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033858
Assay Design:   Intron-spanning
Chromosome Location:   12:104325365-104326180question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000549334 ENST00000299767 ENST00000537375

Gene Information

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction protein folding protein degradation and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al. 2005 [PubMed 16269234]).[supplied by OMIM Aug 2008]

Gene Symbol:   HSP90B1
Gene Name:   heat shock protein 90kDa beta (Grp94), member 1
Aliases:   ECGP, GP96, GRP94, TRA1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000166598
Entrez:   7184
Chromosome Mapping:   12q24.2-q24.3

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