PrimePCR™ Probe Assay: RXRB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RXRB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RXRB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040012
Assay Design:   Intron-spanning
Chromosome Location:   6:33164338-33165603question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000552822 ENST00000550731 ENST00000531100 ENST00000436753 ENST00000443603 ENST00000383217 ENST00000383216 ENST00000546468 ENST00000552454 ENST00000306270 ENST00000377636 ENST00000431480 ENST00000377625 ENST00000425511 ENST00000409546 ENST00000374685 ENST00000374680 ENST00000544186 ENST00000413614 ENST00000410105 ENST00000481739 ENST00000540193 ENST00000455462 ENST00000415157 ENST00000547763 ENST00000546472 ENST00000431161 ENST00000415909 ENST00000549662 ENST00000548862 ENST00000375001 ENST

Gene Information

This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid (RA). This receptor forms homodimers with the retinoic acid thyroid hormone and vitamin D receptors increasing both DNA binding and transcriptional function on their respective response elements. The gene lies within the major histocompatibility complex (MHC) class II region on chromosome 6. An alternatively spliced transcript variant has been described but its full length sequence has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   RXRB
Gene Name:   retinoid X receptor, beta
Aliases:   DAUDI6, H-2RIIBP, MGC1831, NR2B2, RCoR-1
RefSeq:   NC_000006.11 NG_011589.1 NG_023374.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204231
Entrez:   6257
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.820000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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