PrimePCR™ Probe Assay: PRKCZ, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: PRKCZ, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRKCZ, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040542
Assay Design:   Intron-spanning
Chromosome Location:   1:2077469-2080342question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000378567 ENST00000400921 ENST00000461106 ENST00000461465 ENST00000470596 ENST00000496325 ENST00000482686 ENST00000486681 ENST00000470986 ENST00000470511 ENST00000471018 ENST00000497183 ENST00000400920

Gene Information

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   PRKCZ
Gene Name:   protein kinase C, zeta
Aliases:   PKC-ZETA, PKC2
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000067606
Entrez:   5590
Chromosome Mapping:   1p36.33-p36.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 34.300000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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