PrimePCR™ Probe Assay: CFLAR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CFLAR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CFLAR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041627
Assay Design:   Intron-spanning
Chromosome Location:   2:201997826-202000808question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000309955 ENST00000443227 ENST00000341222 ENST00000341582 ENST00000342795 ENST00000395148 ENST00000423241 ENST00000440180 ENST00000457277 ENST00000494258 ENST00000470178 ENST00000462763 ENST00000479953 ENST00000355558 ENST00000340870

Gene Information

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene and partial evidence for several more variants exists. [provided by RefSeq Feb 2011]

Gene Symbol:   CFLAR
Gene Name:   CASP8 and FADD-like apoptosis regulator
Aliases:   CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000003402
Entrez:   8837
Chromosome Mapping:   2q33-q34

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