PrimePCR™ Probe Assay: Sphk1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Sphk1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Sphk1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0042692
Assay Design:   exonic
Chromosome Location:   11:116535088-116535256question
Amplicon Length:   61
Splice Variants Targeted:   ENSMUST00000106388 ENSMUST00000138840 ENSMUST00000106387 ENSMUST00000100201 ENSMUST00000106386 ENSMUST00000145737 ENSMUST00000155102 ENSMUST00000063446 ENSMUST00000063396 ENSMUST00000154034 ENSMUST00000124682 ENSMUST00000141798

Gene Information

This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate which is involved in cell differentiation motility and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq Feb 2010]

Gene Symbol:   Sphk1
Gene Name:   Sphingosine kinase 1
Aliases:   1110006G24Rik, Sk1, Spk1
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000061878
Entrez:   20698
Chromosome Mapping:   11 E2|11

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